Journal: PLoS ONE
Article Title: Integrative Discovery of Epigenetically Derepressed Cancer Testis Antigens in NSCLC
doi: 10.1371/journal.pone.0008189
Figure Lengend Snippet: (A) Schematic outline of the integrative epigenetic screening strategy utilized in this study which combined the pharmacologic demethylation of normal cell lines, comparative COPA anaylsis in primary tissue and correlation of gene expression with the epigenetic regulator BORIS in primary tissue. (B) Representative COPA graph of MAGEA12 demonstrating the statistical approach used to find candidate overexpressed CTAs and related genes. Difference in tumor versus normal expression was significant, p value<0.00001 as measured by Mann-Whitney U test. (C) Upfold regulation of mRNA expression in treated normal lung cell lines, NHBE and SAEC, as measured by Affymetrix Human Genome U133 Plus 2.0 mRNA expression platform.
Article Snippet: We carried out oligonucleotide microarray analysis using the GeneChip U133 Plus 2.0 Affymetrix expression microarray (Affymetrix, Santa Clara, CA).
Techniques: Expressing, MANN-WHITNEY